Solanki, Pratima Rathee and Kaushik, Ajeet and Manaka, T. and Pandey, Manoj Kumar and Iwamoto, M. and Agrawal, Ved Varun and Malhotra, Bansi Dhar (2010) Self-assembled monolayer based impedimetric platform for food borne mycotoxin detection. Nanoscale .
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A self-assembled monolayer (SAM) of 11-amino-1-undecanethiol (AUT) has been fabricated onto a gold (Au) substrate to co-immobilize anti-ochratoxin-A antibodies (AO-IgGs) and bovine serum albumin (BSA) to detect food borne mycotoxin [i.e., ochratoxin-A (OTA)]. AUT/Au electrode, AO-IgGs/AUT/Au immunoelectrode and BSA/IgGs/AUT/Au immunoelectrode have been characterized using scanning electron microscopy (SEM) and electrochemical studies such as cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Electrochemical studies reveal that the AUT-SAM with NH2 groups provide favorable conditions to immobilize AO-IgGs with better orientation, resulting in enhanced electron transport to obtain improved sensing characteristics. The EIS response studies of the BSA/AO-IgGs/AUT/Au immunoelectrode obtained as a function of OTA concentration reveal that the value of the charge transfer resistance (RCT) increases with increased OTA concentration. The BSA/AO-IgGs/AUT/Au immunoelectrode exhibits linearity over 0.5–6.0 ng/dl, detection limit of 0.08 ng/dl using 3sb/m criteria, response time of 30 s and sensitivity of 36.83 U/ng dl 1 cm 2 with a regression coefﬁcient of 0.999. Attempts have been made to monitor the change in RCT of BSA/AO-IgGs/AUT/Au immunoelectrode on addition of coffee samples.
|Subjects:||Material Science > Nanochemistry|
|Deposited On:||28 Nov 2010 15:13|
|Last Modified:||28 Nov 2010 15:14|
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