Uzun, K. and evik, E. and enel, M. and zeri, H. and Baykal, A. and Abasıyanık, M. F. and Toprak, M. S. (2010) Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles. Journal of Nanoparticle Research, 12 (8). pp. 3057-3067.
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In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of super-paramagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modiﬁed with only amino silane. Maximum reaction rate (Vmax) and Michaelis Menten constant (Km) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited On:||14 Jan 2011 06:47|
|Last Modified:||14 Jan 2011 06:47|
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