Nano Archive

Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

Garcia-Cordero, Jose L. and Barrett, Louise M. and O’Kennedy, Richard and Ricco, Antonio J. (2010) Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring. Biomedical Microdevices, 12 (6). pp. 1051-1059.

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Official URL: http://www.springerlink.com/content/b621749g505r56...

Abstract

We report a rapid, low-cost, portable microfluidic sedimentation cytometer (SeCy) for assessing the somatic cell count and fat content of milk in 15 min using a “sample-in, answer-out” approach. The system consists of 12 independent microfluidic devices, essentially flattened funnel structures, fabricated on the footprint of a single plastic compact disc (CD). Each funnel structure holds 150 μL of milk, has an inlet for milk filling and an outlet for air to escape, and ends in a narrow, closed-end microfluidic channel that facilitates packing of the cells into a column whose length is proportional to cell count. The closed-end channel provides accurate cell counts over the range 50,000–>3,000,000 cells per mL. The assay separates cells and fat globules based on their densities (by differential sedimentation), concentrating white cells in the closed-end channel near the outer rim of the CD for estimation of total “cell pellet” volume, while fat globules move toward the center of disc rotation, forming a fat “band” in the funnel. After adding milk to two or more microfluidic devices, the CD is loaded onto a custom-built reader unit that spins the disc for 15 min. Two low-cost microscopes in the reader image the centrifuged cell pellet and the fat band, providing a sufficiently accurate cell count to diagnose mastitis and measuring fat content as an indication of health and nutritional status.

Item Type:Article
Subjects:Physical Science > Nanophysics
Physical Science > Nano objects
Material Science > Nanochemistry
Material Science > Nanostructured materials
Divisions:Faculty of Engineering, Science and Mathematics > School of Physics
Faculty of Engineering, Science and Mathematics > School of Chemistry
ID Code:9887
Deposited By:JNCASR
Deposited On:26 Nov 2010 05:41
Last Modified:26 Nov 2010 05:41

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