Zhu, XS and Gu, Y (2010) Modified nano-TiO2 coupled with fluorescence spectroscopy for the separation/analysis of L-tryptophan. ANALYTICAL BIOCHEMISTRY . ISSN 0003-2697
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A novel adsorbent of carboxymethyl-beta-cyclodextrin modified nanometer TiO2 (CM-beta-CD/TiO2) was prepared and used as a solid-phase extraction (SPE) material coupled to fluorescence spectroscopy determination of L-tryptophan (L-Trp) in biological samples. The experimental conditions for modified nanometer TiO2 separation/preconcentration of L-Trp were optimized. The adsorption capacity of CM-beta-CD/TiO2 for L-Trp was 75.2 mu g. The linear range, detection limit (DL), and the relative standard deviation (RSD) were 0.10-1.20 mu g/mL, 18.8 ng/mL, and 0.67% (n = 3, 1.0 mu g/mL), respectively, with a preconcentration factor of 10. The developed method was applied to determination of L-Trp in real samples and the recoveries were found to be in the range of 99.2-100.3%. For validation, a comparison material of NIC-140686 sample was analyzed and the determined value was in good agreement with the certified value.
|Subjects:||Material Science > Bio materials|
|Deposited On:||28 Oct 2010 10:31|
|Last Modified:||28 Oct 2010 10:31|
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