Qian, Z and Bai, HJ and Wang, GL and Xu, JJ and Chen, HY (2010) A photoelectrochemical sensor based on CdS-polyamidoamine nano-composite film for cell capture and detection. BIOSENSORS & BIOELECTRONICS . ISSN 0956-5663
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We demonstrated herein a newly developed photoelectrochemical cell-sensor for the determination of SMMC-7721 human hepatoma carcinoma cells (SMMC-7721 cells) by using a photosensitive CdS-polyamidoamine (G4) nano-composite film (CdS-PAMAM). The film was generated by electrodeposition method. The presence of PAMAM in the film eliminated the surface defects of CdS nanoparticles and therefore resulted in a greatly enhanced photocurrent and a reduced dark current. In the presence of the electron donor ascorbic acid (AA), the photoexcitation of this modified electrode potentiostated at 0 V versus Ag/AgCl led to an anodic photocurrent. As a result of the covalent coupling reactions, a layer of concanavalin A (ConA) was firmly bound to the functionalized CdS-PAMAM film via glutaraldehyde bridges. The resulting modified electrodes were tested as sensors for SMMC-7721 cell capture and detection via affinity interactions between ConA and mannosyl groups on cell surface. The cell concentration was measured from 5.0 x 10(3) to 1.0 x 10(7) cells through the decrease in photocurrent intensity resulting from its specific binding onto the photosensitive film, the detection limit being 5.0 x 10(3) cells mL(-1).
|Subjects:||Material Science > Nanochemistry|
|Deposited On:||25 Sep 2010 12:35|
|Last Modified:||25 Sep 2010 12:35|
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