Nano Archive

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Cai, Miao and Li, Feng and Zhang, Yan and Wang, Qiangbin (2010) One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection. Nano Research, 3 (8). pp. 557-563. ISSN 1998-0124 (Print) 1998-0000 (Online)

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Official URL: http://www.springerlink.com/content/nq0k772k5p6357...

Abstract

We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:9517
Deposited By:M T V
Deposited On:23 Aug 2010 14:25
Last Modified:23 Aug 2010 14:25

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