Nano Archive

Patterned PDMS based cell array system: a novel method for fast cell array fabrication

Hsieh, Chin-Hsiung and Huang, Chi-Jer Charles and Huang, Yi-You (2010) Patterned PDMS based cell array system: a novel method for fast cell array fabrication. Biomedical Microdevices, 12 (5). pp. 897-905.

[img]
Preview
PDF
686Kb

Official URL: http://www.springerlink.com/content/330440154707u5...

Abstract

Cell-cell interaction is important in numerous biological processes, including cell growth, cell differentiation and migration. The ability to generate pre-determined cell patterns or cell arrays on a study surface is crucial for cell-cell interaction studies. In this paper, we developed a method for fast cell array fabrication using laser sintering and the hydrophobicity of PDMS films. This approach can be easily adopted and is cost-effective. Hydrophobic PDMS films were fabricated into polymeric chips containing hundreds of microwells. The films were then transferred onto tissue culture surfaces to separate cells in the formation of cell arrays (Patterned PDMS based cell array system, PCAS). We used NIH/3T3 fibroblast cells to demonstrate the feasibility of PCAS. The success of fast fabrication of patterned cell arrays was obtained using different initial cell seeding densities. We also used poorly adherent PC-12 cells to demonstrate the cell-cell communication. Results showed that the method is very useful for studying topics such as cell-cell interaction, cell-substrate interaction or cell migration.

Item Type:Article
Subjects:Physical Science > Nanophysics
Physical Science > Nano objects
Material Science > Nanochemistry
Material Science > Nanostructured materials
Divisions:Faculty of Engineering, Science and Mathematics > School of Physics
Faculty of Engineering, Science and Mathematics > School of Chemistry
ID Code:9497
Deposited By:JNCASR
Deposited On:22 Sep 2010 06:52
Last Modified:22 Sep 2010 06:52

Repository Staff Only: item control page