Integrated microfluidic biophotonic chip for laser induced fluorescence detection

Abstract

Integrated Lab-on-a-Chip or Micro-Total Analysis Systems offer several advantages for the detection of active chemical and biological species. In this work, an integrated microfluidic biophotonic chip is proposed for carrying out laser induced fluorescence detection. A Spectrometer-on-Chip device, specifically designed for multiple fluorescence detections at different emission wavelengths is integrated with the opto-microfluidic chip fabricated on Silicon-Polymer hybrid platform. The input fiber from the laser source, and output fiber coupled with a Spectrometer-on-Chip were integrated with the microfluidic channel so as to make a robust setup. Fluorescence detection was carried out using Alexafluor 647 tagged antibody particles. The experimental results show that the proposed biophotonic microfluidic device is highly suitable for high throughput detection of chemical and biological specimens.