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Multiphysics simulation of a microfluidic perfusion chamber for brain slice physiology

Caicedo, Hector H. and Hernandez, Maximiliano and Fall, Christopher P. and Eddington, David T. (2010) Multiphysics simulation of a microfluidic perfusion chamber for brain slice physiology. BIOMEDICAL MICRODEVICES, 12 (5). pp. 761-767. ISSN 1387-2176 (Print) 1572-8781 (Online)

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Official URL: http://www.springerlink.com/content/k1p46q83163052...

Abstract

Understanding and optimizing fluid flows through in vitro microfluidic perfusion systems is essential in mimicking in vivo conditions for biological research. In a previous study a microfluidic brain slice device (μBSD) was developed for microscale electrophysiology investigations. The device consisted of a standard perfusion chamber bonded to a polydimethylsiloxane (PDMS) microchannel substrate. Our objective in this study is to characterize the flows through the μBSD by using multiphysics simulations of injections into a pourous matrix to identify optimal spacing of ports. Three-dimensional computational fluid dynamic (CFD) simulations are performed with CFD-ACE + software to model, simulate, and assess the transport of soluble factors through the perfusion bath, the microchannels, and a material that mimics the porosity, permeability and tortuosity of brain tissue. Additionally, experimental soluble factor transport through a brain slice is predicted by and compared to simulated fluid flow in a volume that represents a porous matrix material. The computational results are validated with fluorescent dye experiments.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:9490
Deposited By:M T V
Deposited On:10 Aug 2010 15:15
Last Modified:16 Aug 2010 16:09

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