Choi, Yoonsu and Yuen, Carrie and Maiti, Sourindra N. and Olivares, Simon and Gibbons, Hillary and Huls, Helen and Raphael, Robert and Killian, Thomas C. and Stark, Daniel J. and Lee, Dean A. and Torikai, Hiroki and Monticello, Daniel and Kelly, Susan S. and Kebriaei, Partow and Champlin, Richard E. and Biswal, Sibani L. and Cooper, Laurence J. N. (2010) A high throughput microelectroporation device to introduce a chimeric antigen receptor to redirect the specificity of human T cells. BIOMEDICAL MICRODEVICES, 12 (5). pp. 855-863. ISSN 1387-2176 (Print) 1572-8781 (Online)
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Official URL: http://www.springerlink.com/content/t7l154k18lh422...
It has been demonstrated that a chimeric antigen receptor (CAR) can directly recognize the CD19 molecule expressed on the cell surface of B-cell malignancies independent of major histocompatibility complex (MHC). Although T-cell therapy of tumors using CD19-specific CAR is promising, this approach relies on using expression vectors that stably integrate the CAR into T-cell chromosomes. To circumvent the potential genotoxicity that may occur from expressing integrating transgenes, we have expressed the CD19-specific CAR transgene from mRNA using a high throughput microelectroporation device. This research was accomplished using a microelectroporator to achieve efficient and high throughput non-viral gene transfer of in vitro transcribed CAR mRNA into human T cells that had been numerically expanded ex vivo. Electro-transfer of mRNA avoids the potential genotoxicity associated with vector and transgene integration and the high throughput capacity overcomes the expected transient CAR expression, as repeated rounds of electroporation can replace T cells that have lost transgene expression. We fabricated and tested a high throughput microelectroporator that can electroporate a stream of 2 × 108 primary T cells within 10 min. After electroporation, up to 80% of the passaged T cells expressed the CD19-specific CAR. Video time-lapse microscopy (VTLM) demonstrated the redirected effector function of the genetically manipulated T cells to specifically lyse CD19+ tumor cells. Our biomedical microdevice, in which T cells are transiently and safely modified to be tumor-specific and then can be re-infused, offers a method for redirecting T-cell specificity, that has implications for the development of adoptive immunotherapy.
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited By:||M T V|
|Deposited On:||10 Aug 2010 15:49|
|Last Modified:||16 Aug 2010 15:58|
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