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Probing Specific Interaction Forces Between Human IgG and Rat Anti-Human IgG by Self-Assembled Monolayer and Atomic Force Microscopy

Lv, Zhengjian and Wang, Jianhua and Chen, Guoping and Deng, Linhong (2010) Probing Specific Interaction Forces Between Human IgG and Rat Anti-Human IgG by Self-Assembled Monolayer and Atomic Force Microscopy. NANOSCALE RESEARCH LETTERS, 5 (6). pp. 1032-1038. ISSN 1931-7573 (Print) 1556-276X (Online)

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Official URL: http://www.springerlink.com/content/06632225x72697...

Abstract

Interaction forces between biological molecules such as antigen and antibody play important roles in many biological processes, but probing these forces remains technically challenging. Here, we investigated the specific interaction and unbinding forces between human IgG and rat anti-human IgG using self assembled monolayer (SAM) method for sample preparation and atomic force microscopy (AFM) for interaction force measurement. The specific interaction force between human IgG and rat anti-human IgG was found to be 0.6–1.0 nN, and the force required for unbinding a single pair of human IgG and rat anti-human IgG was calculated to be 144 ± 11 pN. The results are consistent with those reported in the literatures. Therefore, SAM for sample preparation combined with AFM for interaction measurement is a relatively simple, sensitive and reliable technique to probe specific interactions between biological molecules such as antigen and antibody.

Item Type:Article
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanotechnology for human health
ID Code:9274
Deposited By:M T V
Deposited On:03 Jun 2010 14:48
Last Modified:03 Jun 2010 14:48

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