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Genomics-based screening of differentially expressed genes in the brains of mice exposed to silver nanoparticles via inhalation

Lee, Hye-Young and Choi, You-Jin and Jung, Eun-Jung and Yin, Hu-Quan and Kwon, Jung-Taek and Kim, Ji-Eun and Im, Hwang-Tae and Cho, Myung-Haing and Kim, Ju-Han and Kim, Hyun-Young and Lee, Byung-Hoon (2010) Genomics-based screening of differentially expressed genes in the brains of mice exposed to silver nanoparticles via inhalation. Journal of Nanoparticle Research, 12 (5). pp. 1567-1578.

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Abstract

Silver nanoparticles (AgNP) are among the fastest growing product categories in the nanotechnology industry. Despite the importance of AgNP in consumer products and clinical applications, relatively little is known regarding AgNP toxicity and its associated risks. We investigated the effects of AgNP on gene expression in the mouse brain using Affymetrix Mouse Genome Arrays. C57BL/6 mice were exposed to AgNP (geometric mean diameter, 22.18 ± 1.72 nm; 1.91 × 107 particles/cm3) for 6 h/day, 5 days/week using the nose-only exposure system for 2 weeks. Total RNA isolated from the cerebrum and cerebellum was subjected to hybridization. From over 39,000 probe sets, 468 genes in the cerebrum and 952 genes in the cerebellum were identified as AgNP-responsive (one-way analysis of variance; p < 0.05). The largest groups of gene products affected by AgNP exposure included 73 genes in the cerebrum and 144 genes in the cerebellum. AgNP exposure modulated the expression of several genes associated with motor neuron disorders, neurodegenerative disease, and immune cell function, indicating potential neurotoxicity and immunotoxicity associated with AgNP exposure. Real-time PCR data for five genes analyzed from whole blood showed good correlation with the observed changes in the brain. Following rigorous validation and substantiation, these genes may assist in the development of surrogate markers for AgNP exposure and/or toxicity.

Item Type:Article
Uncontrolled Keywords:Brain - Inhalation - Mice - Microarray - Silver nanoparticles - Toxicity - EHS - Nanomedicine
Subjects:Biomedical Science > Nanotechnology for human health
NanoSafety > Environment, health and safety aspects of nanotechnology
ID Code:9266
Deposited By:SPI
Deposited On:08 Jun 2010 17:58
Last Modified:08 Jun 2010 17:58

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