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Synthesis of bombesin-functionalized iron oxide nanoparticles and their specific uptake in prostate cancer cells

Martin, Amanda L. and Hickey, Jennifer L. and Ablack, Amber L. and Lewis, John D. and Luyt, Leonard G. and Gillies, Elizabeth R. (2010) Synthesis of bombesin-functionalized iron oxide nanoparticles and their specific uptake in prostate cancer cells. Journal of Nanoparticle Research, 12 (5). pp. 1599-1608.

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Abstract

The imaging of molecular markers associated with disease offers the possibility for earlier detection and improved treatment monitoring. Receptors for gastrin-releasing peptide are overexpressed on prostate cancer cells offering a promising imaging target, and analogs of bombesin, an amphibian tetradecapeptide have been previously demonstrated to target these receptors. Therefore, the pan-bombesin analog [β-Ala11, Phe13, Nle14]bombesin-(7–14) was conjugated through a linker to dye-functionalized superparamagnetic iron oxide nanoparticles for the development of a new potential magnetic resonance imaging probe. The peptide was conjugated via click chemistry, demonstrating a complementary alternative methodology to conventional peptide-nanoparticle conjugation strategies. The peptide-functionalized nanoparticles were then demonstrated to be selectively taken up by PC-3 prostate cancer cells relative to unfunctionalized nanoparticles and this uptake was inhibited by the presence of free peptide, confirming the specificity of the interaction. This study suggests that these nanoparticles have the potential to serve as magnetic resonance imaging probes for the detection of prostate cancer.

Item Type:Article
Uncontrolled Keywords:Iron oxide - Nanoparticles - Bombesin - Prostate cancer - Magnetic resonance imaging - Nanomedicine
Subjects:Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:9263
Deposited By:SPI
Deposited On:08 Jun 2010 17:56
Last Modified:08 Jun 2010 17:56

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