Liu, Lanxia and Bai, Yuanyuan and Song, Chunni and Zhu, Dunwan and Song, Liping and Zhang, Hailing and Dong, Xia and Leng, Xigang (2010) The impact of arginine-modified chitosan–DNA nanoparticles on the function of macrophages. Journal of Nanoparticle Research, 12 (5). pp. 1637-1644.
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It has been demonstrated that incorporation of arginine moieties into chitosan significantly elevates the transgenic efficacy of the chitosan. However, little is known about the impact of arginine-modified chitosan on the function of macrophages, which play a vitally important role in the inflammatory response of the body to foreign substances, especially particulate substances. This study was designed to investigate the impact of arginine-modified chitosan/DNA nanoparticles on the function of the murine macrophage through observation of phagocytic activity and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10, IL-12, and TNF-α). Results showed that both chitosan/DNA nanoparticles and arginine-modified chitosan/DNA nanoparticles, containing 20 μg/mL DNA, were internalized by almost all the macrophages in contact. This led to no significant changes, compared to the non-exposure group, in production of cytokines and phagocytic activity of the macrophages 24 h post co-incubation, whereas exposure to LPS induced obviously elevated cytokine production and phagocytic activity, suggesting that incorporation of arginine moieties into chitosan does not have a negative impact on the function of the macrophages.
|Uncontrolled Keywords:||DNA nanoparticle - Macrophages - Cytokines - Chitosan - Arginine - Nanobiotechnology|
|Subjects:||Biomedical Science > Nanobiotechnology|
Biomedical Science > Nanomedicine
|Deposited On:||08 Jun 2010 17:54|
|Last Modified:||08 Jun 2010 17:54|
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