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The impact of arginine-modified chitosan–DNA nanoparticles on the function of macrophages

Liu, Lanxia and Bai, Yuanyuan and Song, Chunni and Zhu, Dunwan and Song, Liping and Zhang, Hailing and Dong, Xia and Leng, Xigang (2010) The impact of arginine-modified chitosan–DNA nanoparticles on the function of macrophages. Journal of Nanoparticle Research, 12 (5). pp. 1637-1644.

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Abstract

It has been demonstrated that incorporation of arginine moieties into chitosan significantly elevates the transgenic efficacy of the chitosan. However, little is known about the impact of arginine-modified chitosan on the function of macrophages, which play a vitally important role in the inflammatory response of the body to foreign substances, especially particulate substances. This study was designed to investigate the impact of arginine-modified chitosan/DNA nanoparticles on the function of the murine macrophage through observation of phagocytic activity and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10, IL-12, and TNF-α). Results showed that both chitosan/DNA nanoparticles and arginine-modified chitosan/DNA nanoparticles, containing 20 μg/mL DNA, were internalized by almost all the macrophages in contact. This led to no significant changes, compared to the non-exposure group, in production of cytokines and phagocytic activity of the macrophages 24 h post co-incubation, whereas exposure to LPS induced obviously elevated cytokine production and phagocytic activity, suggesting that incorporation of arginine moieties into chitosan does not have a negative impact on the function of the macrophages.

Item Type:Article
Uncontrolled Keywords:DNA nanoparticle - Macrophages - Cytokines - Chitosan - Arginine - Nanobiotechnology
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:9260
Deposited By:SPI
Deposited On:08 Jun 2010 17:54
Last Modified:08 Jun 2010 17:54

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