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Multiplexed five-color molecular imaging of cancer cells and tumor tissues with carbon nanotube Raman tags in the near-infrared

Liu, Zhuang and Tabakman, Scott and Sherlock, Sarah and Li, Xiaolin and Chen, Zhuo and Jiang, Kaili and Fan, Shoushan and Dai, Hongjie (2010) Multiplexed five-color molecular imaging of cancer cells and tumor tissues with carbon nanotube Raman tags in the near-infrared. Nano Research, 3 (3). pp. 222-233.

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Official URL: http://www.springerlink.com/content/w766wx58765866...

Abstract

Single-walled carbon nanotubes (SWNTs) with five different C13/C12 isotope compositions and well-separated Raman peaks have been synthesized and conjugated to five targeting ligands in order to impart molecular specificity. Multiplexed Raman imaging of live cells has been carried out by highly specific staining of cells with a five-color mixture of SWNTs. Ex vivo multiplexed Raman imaging of tumor samples uncovers a surprising up-regulation of epidermal growth factor receptor (EGFR) on LS174T colon cancer cells from cell culture to in vivo tumor growth. This is the first time five-color multiplexed molecular imaging has been performed in the near-infrared (NIR) region under a single laser excitation. Near zero interfering background of imaging is achieved due to the sharp Raman peaks unique to nanotubes over the low, smooth autofluorescence background of biological species.

Item Type:Article
Uncontrolled Keywords:Carbon nanotubes - Raman scattering - biomedicine - multiplexed imaging
Subjects:Material Science > Functional and hybrid materials
Biomedical Science > Nanobiotechnology
Material Science > Soft materials
Biomedical Science > Nanomedicine
ID Code:9015
Deposited By:Lesley Tobin
Deposited On:27 Jul 2010 12:45
Last Modified:27 Jul 2010 12:45

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