Nano Archive

Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress

Zorlutuna, P. and Rong, Z. and Vadgama, P. and Hasirci, V. (2009) Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress. Acta Biomaterialia, 5 (7). 2451 - 2459.

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In this study, nanopatterned crosslinked films of collagen Type I were seeded with human microvascular endothelial cells and tested for their suitability for vascular tissue engineering. Since the films will be rolled into tubes with concentric layers of collagen, nutrient transfer through the collagen films is quite crucial. Molecular diffusivity through the collagen films, cell viability, cell proliferation and cell retention following shear stress were studied. Cells were seeded onto linearly nanogrooved films (groove widths of 332.5, 500 and 650 nm), with the grooves aligned in the direction of flow. The nanopatterns did not affect cell proliferation or initial cell alignment; however, they significantly affected cell retention under fluid flow. While cell retention on unpatterned films was 35 ± 10%, it was 75 ± 4% on 332.5 nm patterned films and even higher, 91 ± 5%, on 650 nm patterned films. The films were found to have diffusion coefficients of ca. 10−6 cm2 s−1 for O2 and 4-acetaminophenol, which is comparable to that observed in natural tissues. This constitutes another positive asset of these films for consideration as a scaffold material for vascular tissue engineering.

Item Type:Article
Uncontrolled Keywords:Shear stress; HMEC; Collagen; Nanopattern; Vascular tissue engineering
Subjects:Biomedical Science > Nanomedicine
ID Code:8979
Deposited By:SPI
Deposited On:04 May 2010 10:26
Last Modified:04 May 2010 10:26

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