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Monosize magnetic hydrophobic beads for lysozyme purification under magnetic field

AltIntas, Evrim Banu and Denizli, Adil (2009) Monosize magnetic hydrophobic beads for lysozyme purification under magnetic field. Materials Science and Engineering: C, 29 (5). 1627 - 1634.

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Official URL: http://www.sciencedirect.com/science/article/B6TXG...

Abstract

Monosize and magnetic poly(glycidyl methacrylate-N-methacryloyl-(l)-tryptophan) [mPGMATrp] beads (1.6 µm in diameter) were used for hydrophobic affinity capture of lysozyme from chicken egg-white. N-methacryloyl-(l)-tryptophan (MATrp), which gives hydrophobicity to the resulting polymer, was synthesized by reacting methacryloyl chloride and l-tryptophan methyl ester then characterized by Nuclear Magnetic Resonance (NMR). mPGMATrp beads were produced by dispersion polymerization in the presence of magnetite nano-powder. mPGMATrp beads were characterized by means of swelling studies, elemental analysis, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). Lysozyme adsorption experiments were performed under different experimental conditions (i.e., lysozyme concentration, temperature, and ionic strength) in magnetically stabilized fluidized bed system, (MSFB). Maximum adsorption capacity was 263.9 mg/g. It was observed that mPGMATrp beads can be used without significant loss in lysozyme adsorption capacity after 25 adsorption-elution cycle.

Item Type:Article
Uncontrolled Keywords:Protein purification; Hydrophobic interaction chromatography; Lysozyme; Magnetic beads
Subjects:Physical Science > Nanomagnetics
ID Code:8974
Deposited By:SPI
Deposited On:03 May 2010 17:24
Last Modified:03 May 2010 17:24

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