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Internalization and cytotoxicity analysis of silicon-based microparticles in macrophages and embryos

Fernández-Rosas, Elisabet and Gómez, Rodrigo and Ibañez, Elena and Barrios, Lleonard and Duch, Marta and Esteve, Jaume and Plaza, José and Nogués, Carme (2010) Internalization and cytotoxicity analysis of silicon-based microparticles in macrophages and embryos. Biomedical Microdevices, 12 (3). pp. 371-379.

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Official URL: http://www.springerlink.com/content/w362966t313687...

Abstract

Microchips can be fabricated, using semiconductor technologies, at microscopic level to be introduced into living cells for monitoring of intracellular parameters at a single cell level. As a first step towards intracellular chips development, silicon and polysilicon microparticles of controlled shape and dimensions were fabricated and introduced into human macrophages and mouse embryos by phagocytosis and microinjection, respectively. Microparticles showed to be non-cytotoxic for macrophages and were found to be localized mainly inside early endosomes, in tight association with endosomal membrane, and more rarely in acidic compartments. Embryos with microinjected microparticles developed normally to the blastocyst stage, confirming the non-cytotoxic effect of the particles. In view of these results silicon and polysilicon microparticles can serve as the frame for future intracellular chips development and this technology opens the possibility of real complex devices to be used as sensors or actuators inside living cells.

Item Type:Article
Uncontrolled Keywords:Silicon - Polysilicon - Microparticle - MEMS - Cytotoxicity
Subjects:Biomedical Science > Nanobiotechnology
Physical Science > Nanoelectronics
Material Science > Bio materials
NanoSafety > Environment, health and safety aspects of nanotechnology
Biomedical Science > Nanotechnology for human health
Material Science > Nanostructured materials
ID Code:8956
Deposited By:IoN
Deposited On:29 Apr 2010 13:40
Last Modified:29 Apr 2010 13:40

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