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A microfluidic imaging chamber for the direct observation of chemotactic transmigration

Breckenridge, Mark T. and Egelhoff, Thomas T. and Baskaran, Harihara (2010) A microfluidic imaging chamber for the direct observation of chemotactic transmigration. Biomedical Microdevices, 12 (3). pp. 543-553.

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To study the roles of nonmuscle myosin II (NM-II) during invasive cell migration, microfluidic migration chambers have been designed and fabricated using photo- and soft-lithography microfabrication techniques. The chamber consists of two channels separated by a vertical barrier with multiple bays of pores with widths varying from 6 µm to 16 µm, and lengths varying from 25 µm to 50 µm. The cells are plated in the channel on one side of the barrier while a chemoattractant is flowed through the channel on the other side of the barrier. In these chambers, cells can be observed with transmitted light or fluorescence optics while they chemotax through various sized pores that impose differential mechanical resistance to transmigration. As an initial test of this device, we compared breast-cancer cell chemotactic transmigration through different pore sizes with and without inhibition of NM-II. Two distinct rates were observed as cells attempted to pull their nucleus through the smaller pores, and the faster nuclear transit mode was critically dependent on NM-II motor activity. The ability to monitor cells as they chemotax through pores of different dimensions within a single experimental system provides novel information on how pore size affects cell morphology and migration rate, providing a dramatic improvement of imaging potential relative to other in vitro transmigration systems such as Boyden chambers.

Item Type:Article
Uncontrolled Keywords:Chemotaxis - Transmigration - Nonmuscle myosin II
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanobiotechnology
Biomedical Science > Nanotechnology for human health
ID Code:8939
Deposited By:IoN
Deposited On:06 May 2010 16:11
Last Modified:06 May 2010 16:11

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