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Electron microscopic estimation of removal of parvovirus B19 (HPVB19) by nanofiltration with a novel filter membrane

Yamaguchi, Kazuhito and Miyagawa, Eiji and Takahashi, Hirohiko and Miyazaki, Toru and Ikeda, Hisami (2007) Electron microscopic estimation of removal of parvovirus B19 (HPVB19) by nanofiltration with a novel filter membrane. JOURNAL OF MEMBRANE SCIENCE, 298 (1-2). pp. 99-109.

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To prevent the risk of infection by infusions of plasma products contaminated with infectious agents in medical treatments., a nanofiltration technique is available to eliminate the agents in the process of plasma manufacture. In this study, the capacity for microparticle removal of the virus filter membrane (Planovao), which consists of hollow fibers (BMMs), for nanofiltration, was estimated by electron microscopy using 20 nm colloidal gold and human parvovirus B 19 (HPVB 19) solutions, and plasma specimens from an HPVB 19-patient. The BMMs used were of 35 nm (BMM35), 20 nm (BMM20) and 15 nm (BMM15) mean pore size. To determine the site at which particles were retained, the BMM wall cut transversely was divided into 10 sections from inner to outer surface of the membrane on the electron micrographs. When gold particle solutions (3.6 x 10(11) particles/0.5 ml) were filtered with 5 mm-length single modules, particles were retained in the section 10, 7, and 8 in the wall of BMM35, BMM20, and BMM15, respectively. When purified HPVB19 suspensions (10(9)-10(13) TCID50-infectivity/ml in 0.5ml) were filtered using the modules, virus particles were observed in the section 10, 5, and 7 in BMM35, BMM20, and BMM15, respectively. After the plasma filtration (10(7) TCID50), virus particles were also observed in the section 8, 4, and 3. Virus particles were identified by immunoelectron microscopy using an anti-HPVB19 monoclonal antibody. In the BMM20 used for HPVB 19 filtration, many immunogold particles were counted on or around the virus observed in the section 5, but the number was drastically reduced and no virus was observed in the outer sections. No infectious HPVB 19 was detected in the filtrates from BMM20 and BMM15. These results demonstrate that BMM20 and BMM 15 can completely remove microparticles larger than 20 nm from the solution and that the smallest human viruses such as HPVB 19 can be removed by using the nanofiltration technique. (c) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:electron microscopy; HPVB 19; microparticle removal; nanofiltration; planova; virus filtration
Subjects:Analytical Science > Microscopy and probe methods
Biomedical Science > Nanotechnology for human health
Material Science > Nanostructured materials
Biomedical Science > Nanomedicine
ID Code:890
Deposited On:04 Dec 2008 17:24
Last Modified:05 Jan 2009 17:12

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