Liu, Yang and Zhang, Deng and Alocilja, Evangelyn C. and Chakrabartty, Shantanu (2010) Biomolecules Detection Using a Silver-Enhanced Gold Nanoparticle-Based Biochip. NANOSCALE RESEARCH LETTERS, 5 (3). pp. 533-538. ISSN 1931-7573 (Print) 1556-276X (Online)
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Official URL: http://www.springerlink.com/content/6k24120128q7h3...
Silver-enhanced labeling method has been employed in immunochromatographic assays for improving the sensitivity of detecting pathogens. In this paper, we apply the silver enhancement technique for biomolecular signal amplification in a gold nanoparticle-based conductimetric biochip. We show that the response of the silver-enhanced biochip comprises two distinct regions namely: (a) a sub-threshold region where conduction occurs due to electron hopping between silver islands and the electrolyte and (b) an above-threshold region where the conduction is due to a direct flow of electrons. These two regions are characterized by different conduction slopes, and we show that combining the information from both these regions can improve the sensitivity of the biochip. Results from fabricated prototypes show a dynamic range of more than 40Â dB and with a detection limit less than 240Â pg/mL. The fabrication of the biochip is compatible with standard complementary metalâoxideâsemiconductor (CMOS) processes making it ideal for integration in next-generation CMOS biosensors.
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited By:||M T V|
|Deposited On:||21 Apr 2010 15:30|
|Last Modified:||21 Apr 2010 15:30|
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