Nano Archive

Biomolecules Detection Using a Silver-Enhanced Gold Nanoparticle-Based Biochip

Liu, Yang and Zhang, Deng and Alocilja, Evangelyn C. and Chakrabartty, Shantanu (2010) Biomolecules Detection Using a Silver-Enhanced Gold Nanoparticle-Based Biochip. NANOSCALE RESEARCH LETTERS, 5 (3). pp. 533-538. ISSN 1931-7573 (Print) 1556-276X (Online)

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Official URL: http://www.springerlink.com/content/6k24120128q7h3...

Abstract

Silver-enhanced labeling method has been employed in immunochromatographic assays for improving the sensitivity of detecting pathogens. In this paper, we apply the silver enhancement technique for biomolecular signal amplification in a gold nanoparticle-based conductimetric biochip. We show that the response of the silver-enhanced biochip comprises two distinct regions namely: (a) a sub-threshold region where conduction occurs due to electron hopping between silver islands and the electrolyte and (b) an above-threshold region where the conduction is due to a direct flow of electrons. These two regions are characterized by different conduction slopes, and we show that combining the information from both these regions can improve the sensitivity of the biochip. Results from fabricated prototypes show a dynamic range of more than 40 dB and with a detection limit less than 240 pg/mL. The fabrication of the biochip is compatible with standard complementary metal–oxide–semiconductor (CMOS) processes making it ideal for integration in next-generation CMOS biosensors.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:8773
Deposited By:M T V
Deposited On:21 Apr 2010 15:30
Last Modified:21 Apr 2010 15:30

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