Nano Archive

Preparation, characterization and application of polyaniline nanospheres to biosensing

Dhand, Chetna and Das, Maumita and Sumana, Gajjala and Srivastava, Avanish Kumar and Pandey, Manoj Kumar and Kim, Cheol Gi and Datta, Monika and Malhotra, Bansi Dhar (2010) Preparation, characterization and application of polyaniline nanospheres to biosensing. nanoscale .

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Polyaniline nanospheres (PANI-NS) prepared by morphological transformation of micelle polymerized camphorsulfonic acid (CSA) doped polyaniline nanotubes (PANI-NT) in the presence of ethylene glycol (EG) have been characterized by X-ray diffraction, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, Fourier transform infra-red and UV-Visible spectroscopy. A PANI-NS (60–80 nm) film deposited onto an indium-tin-oxide (ITO) coated glass plate by the solution casting method has been utilized for covalent immobilization of biomolecules (cholesterol oxidase (ChOx)) via N-ethyl-N -(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry for fabrication of a cholesterol biosensor. The ChOx/PANI-NS/ITO bioelectrode detects cholesterol in the concentration range of 25 to 500 mg dL−1 with sensitivity of 1.3 × 10−3 mA mg−1 dL and regression coefficient of 0.98. Further, this PANI-NS based bioelectrode shows fast response time (10 s), low Michaelis–Menten constant (2.5 mM) and shelf-life of 12 weeks. The spherical nanostructure observed in the final morphology of the PANI-NS film is attributed to hydrogen bonding interactions between PANI-NT and EG.

Item Type:Article
Subjects:Material Science > Nanochemistry
ID Code:8730
Deposited By:CSMNT
Deposited On:27 Mar 2010 06:28
Last Modified:27 Mar 2010 06:28

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