Southworth, Richard and Kaneda, Megan and Chen, Junjie and Zhang, Lei and Zhang, Huiying and Yang, Xiaoxia and Razavi, Reza and Lanza, Gregory and Wickline, Samuel A. (2009) Renal vascular inflammation induced by Western diet in ApoE-null mice quantified by 19F NMR of VCAM-1 targeted nanobeacons. Nanomedicine: Nanotechnology, Biology and Medicine, 5 (3). 359 - 367.
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Official URL: http://www.sciencedirect.com/science/article/B7MDB...
We have designed multifunctional nanoparticulate reporter bioprobes capable of targeting vascular cell adhesion molecule 1 (VCAM-1), which is up-regulated in numerous inflammatory processes. These perfluorocarbon-cored nanoparticles emit a unique 19F magnetic resonance (MR) signature, providing the potential to localize and quantify VCAM-1 expression in early atherosclerosis. Nanoparticle-VCAM-1 targeting specificity was confirmed by in vitro binding and competition studies. ApoE-null and control C57-BL6 mice (n = 6/group), fed a Western diet for 35 weeks, were injected i.v. with targeted or non-targeted nanoparticles. After two hours, kidneys were excised and prepared for analysis. ApoE-null kidneys exhibited increased VCAM-1-targeted nanoparticle content over healthy controls by 19F MR spectroscopy (36.5+8.8 vs. 9.3+2.2 × 108/g, P < .05), which correlated with increased VCAM-1 staining (2.5 ± 1.3% vs. 0.9 ± 0.3%, P < .05); their relative biodistributions were confirmed by fluorescence microscopy and MR imaging. These molecular imaging agents offer new approaches for detection, quantification, and longitudinal evaluation of early inflammation utilising 19F MR spectroscopy and imaging.
|Uncontrolled Keywords:||Molecular imaging; Nanoparticles; Inflammation; VCAM; Magnetic resonance|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited On:||25 Mar 2010 20:03|
|Last Modified:||25 Mar 2010 20:03|
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