Sabnis, Abhimanyu and Wadajkar, Aniket S. and Aswath, Pranesh and Nguyen, Kytai T. (2009) Factorial analyses of photopolymerizable thermoresponsive composite hydrogels for protein delivery. Nanomedicine: Nanotechnology, Biology and Medicine, 5 (3). 305 - 315.
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Official URL: http://www.sciencedirect.com/science/article/B7MDB...
A smart protein delivery system for wound healing applications was developed using composite nanoparticle hydrogels that can release protein in a temperature-responsive manner. This system can also be formed in situ in the presence of ultraviolet light and Irgacure 2959 photoinitiator. The system consists of temperature-sensitive poly(N-isopropylacrylamide-co-acrylamide) (PNIPAM-AAm) nanoparticles embedded in a poly(ethylene glycol) diacrylate (PEGDA) matrix. A factorial analysis was performed to evaluate the effects of PEGDA concentration (10% and 15% w/v) and PEGDA molecular weight (MW; 3.4 kDa and 8 kDa), as well as PNIPAM-AAm nanoparticle concentration (2% and 4% w/v) and temperature (23°C and 40°C) on the protein release profiles and swelling ratios of the hydrogels. Results indicate that PNIPAM-AAm nanoparticle concentration and temperature were the most important factors affecting the protein release during the burst release phase. Additionally, PEGDA MW was the most important factor affecting the protein release in the plateau region. It was also important in controlling the hydrogel swelling ratio. A dual-layered hydrogel was further developed to produce a protein delivery system with a better sustained release. These findings have improved our understanding of the composite hydrogel systems and will help in tailoring future systems with desired release profiles.
|Uncontrolled Keywords:||Photopolymerization; Hydrogel; Thermoresponsiveness; Factorial analysis|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited On:||25 Mar 2010 22:08|
|Last Modified:||25 Mar 2010 22:08|
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