Nyland, Jennifer F. and Bai, Jennifer J. K. and Katz, Howard E. and Silbergeld, Ellen K. (2009) In vitro interactions between splenocytes and dansylamide dye-embedded nanoparticles detected by flow cytometry. Nanomedicine: Nanotechnology, Biology and Medicine, 5 (3). 298 - 304.
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Official URL: http://www.sciencedirect.com/science/article/B7MDB...
Engineered nanoparticles (NPs) possess a range of biological activity. In vitro methods for assessing toxicity and efficacy would be enhanced by simultaneous quantitative information on the behavior of NPs in culture systems and signals of cell response. We have developed a method for visualizing NPs within cells using standard flow-cytometric techniques and uniquely designed spherical siloxane NPs with an embedded (covalently bound) dansylamide dye. This method allowed NP visualization without obscuring detection of relevant biomarkers of cell subtype, activation state, and other events relevant to assessing bioactivity. We determined that NPs penetrated cells and induced a range of biological signals consistent with activation and costimulation. These results indicate that NPs may affect cell function at concentrations below those inducing cytotoxicity or apoptosis and demonstrate a novel method to image both localization of NPs and cell-level effects.
|Uncontrolled Keywords:||Nanotoxicology; In vitro; Flow cytometry; Cellular function|
|Subjects:||Risk > Environment, health and safety aspects of nanotechnology|
Biomedical Science > Nanomedicine
|Deposited On:||22 Apr 2010 11:22|
|Last Modified:||22 Apr 2010 11:22|
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