Niwa, Yasuharu and Iwai, Naoharu (2007) Nanomaterials induce oxidized low-density lipoprotein cellular uptake in macrophages and platelet aggregation. CIRCULATION JOURNAL, 71 (3). pp. 437-444.
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Official URL: http://ci.nii.ac.jp/naid/110006224217/
Background Nanomaterials have numerous potential benefits for society, but the potential hazards of nanomaterials on hurnan health are poorly understood. Nanomaterials are known to pass into the circulatory system in humans, causing vascular injuries that inight play a role in the development of atherosclerosis. The present study aimed to determine the effects of chronic exposure to nanornaterials on macrophage phenotype and platelet aggregation. Methods and Results Cultured macrophages (RAW264.7) were treated with carbon black (CB) and watersoluble fullerene (C-60(OH)(24)) from 7 to 50 days. Individually, CB had no significant effects on RAW264.7 cell growth, whereas C-60(OH)(24) alone or CB and C-60(OH)(24) together with oxidized low-density lipoprotein (alpha-LDL) (100 mu g/ml) induced cytotoxic morphological changes, Such as Ox-LDL uptake-induced foarn cell-like formation and decreased cell growth, in a dose-dependent manner. C60(OH)24 induced LOX-1 protein expression, pro-matrix metalloprotease-9 protein secretion, and fissue factor mRNA expression in lipid-laden macrophages. Although CB or C-60(OH)(24) alone did not induce platelet aggregation, C-60(OH)(24) facilitated adenosine diphosphate (ADP)induced platelet aggregation. Furthermore, C-60(OH)(24) acted as a competitive inhibitor of ADP receptor antagonists in ADP-mediated platelet aggregation. Conclusions The present study confirmed novel effects of nanomaterials in niacrophages and platelets. These effects suggest that exposure to nanomaterials might be a risk for atherothrornbotic diseases.
|Uncontrolled Keywords:||atherosclerosis; lipid-laden macrophages; nanomaterials; platelet aggregation|
|Subjects:||Risk > Environment, health and safety aspects of nanotechnology|
Material Science > Nanostructured materials
|Deposited By:||INVALID USER|
|Deposited On:||05 Dec 2008 15:10|
|Last Modified:||05 Dec 2008 15:10|
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