Nano Archive

Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation

Soman, Chinmay and Giorgio, Todd (2009) Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation. Nanomedicine: Nanotechnology, Biology and Medicine, 5 (4). 402 - 409.

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A rapid, single-step, solution-phase method for quantifying multiple proteomic biomarkers is described. Nanoscale quantum dot–antibody conjugates self-assemble into microscale aggregates in the presence of a specific antigen through antibody-antigen molecular recognition. These aggregates are easily discriminated from the individual components by flow cytometry. Quantum dot (QD) aggregates can be quantified and correlated to the antigen concentration. Two QD populations with distinct emission spectra are used for detecting two proteomics antigens in a single reaction volume. Multiplexed detection of vascular endothelial growth factor A and angiopoietin-2 is demonstrated at the physiologically relevant, picomolar concentration range. Nonmultiplexed detection of the antigens is also demonstrated, with a femtomolar sensitivity limit. This technique may be optimized for low-cost early detection and frequent screening of cancers and other diseases as well as detection of the biological response to therapy.

Item Type:Article
Uncontrolled Keywords:Self-assembly; Quantum dots; Diagnostics; Aggregates; Proteomics
Subjects:Physical Science > Quantum phenomena
Biomedical Science > Nanomedicine
ID Code:8579
Deposited By:SPI
Deposited On:22 Apr 2010 11:04
Last Modified:22 Apr 2010 11:04

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