Yan, Fei and Zhang, Chao and Zheng, Yi and Mei, Lin and Tang, Lina and Song, Cunxian and Sun, Hongfan and Huang, Laiqiang (2010) The effect of poloxamer 188 on nanoparticle morphology, size, cancer cell uptake, and cytotoxicity. Nanomedicine: Nanotechnology, Biology and Medicine, 6 (1). 170 - 178.
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Official URL: http://www.sciencedirect.com/science/article/B7MDB...
The aim of this work was to investigate the effect of triblock copolymer poloxamer 188 on nanoparticle morphology, size, cancer cell uptake, and cytotoxicity. Docetaxel-loaded nanoparticles were prepared by oil-in-water emulsion/solvent evaporation technique using biodegradable poly(lactic-co-glycolic acid) (PLGA) with or without addition of poloxamer 188, respectively. The resulting nanoparticles were found to be spherical with a rough and porous surface. The nanoparticles had an average size of around 200 nm with a narrow size distribution. The in vitro drug-release profile of both nanoparticle formulations showed a biphasic release pattern. An increased level of uptake of PLGA/poloxamer 188 nanoparticles in the docetaxel-resistant MCF-7 TAX30 human breast cancer cell line could be found in comparison with that of PLGA nanoparticles. In addition, the docetaxel-loaded PLGA/poloxamer 188 nanoparticles achieved a significantly higher level of cytotoxicity than that of docetaxel-loaded PLGA nanoparticles and Taxotere (P < .05). In conclusion, the results showed advantages of docetaxel-loaded PLGA nanoparticles incorporated with poloxamer 188 compared with the nanoparticles without incorporation of poloxamer 188 in terms of sustainable release and efficacy in breast cancer chemotherapy.
|Uncontrolled Keywords:||Breast cancer; Cell viability; Docetaxel; Nanoparticles; Poloxamer 188|
|Subjects:||NanoSafety > Environment, health and safety aspects of nanotechnology|
Biomedical Science > Nanomedicine
|Deposited On:||22 Apr 2010 11:33|
|Last Modified:||22 Apr 2010 11:33|
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