Nano Archive

A novel approach to ultrasensitive diagnosis using supramolecular protein nanoparticles

Lee, Sung-Hyun and Lee, Hyewon and Park, Jin-Seung and Choi, Hyoung and Han, Kyung-Yeon and Seo, Hyuk-Seong and Ahn, Keum-Young and Han, Sung-Sik and Cho, Yunjung and Lee, Kee-Hyoung and Lee, Jeewon (2007) A novel approach to ultrasensitive diagnosis using supramolecular protein nanoparticles. FASEB JOURNAL, 21 (7). pp. 1324-1334.

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We report on the ultrasensitive protein nanoprobe system that specifically captures disease marker ( autoantibodies of Type I diabetes in this case) with attomolar sensitivity. The system relies on supramolecular protein nanoparticles that bind a specific antibody [ 65 kDa glutamate decarboxylase ( GAD(65))- specific autoantibody, i.e., the early marker of Type I diabetes]. The ultrasensitive detection of early marker of Type I diabetes during the early phase of pancreatic beta-cell destruction is important because individuals at high risk of developing Type I diabetes can be identified several years before the clinical onset of the ailment. The bacterial expression of chimera genes encoding N-[human ferritin heavy chain (hFTN- H)]::[ specific antigenic epitope]- C produces supramolecular nanoparticles with uniform diameters ( 10 - 15 nm), owing to self-assembly activity of hFTN- H. Each nanoparticle, formed by intermolecular self-assembly between the chimera protein molecules, is subjected to carrying a large number ( presumably, 24) of epitopes with a homogeneous and stable conformation per autoantibody binding, thereby allowing substantial enhancement of sensitivity. The sensitivity was finally boosted to 3 attomolar concentration of the autoantibodies, 4 - 9 orders of magnitude more sensitive than conventional immunoassays. Also, this ultrasensitive protein nanoprobe successfully detected natural autoantibodies in the sera from Type I diabetic patients. The attomolar sensitivity was successfully reproduced on the detection of other antibodies, i.e., monoclonal antibodies against hepatitis B surface antigen. With the two antibody markers above, the feasibility of simultaneous and multiplexing-mode detection was also demonstrated.

Item Type:Article
Uncontrolled Keywords:human ferritin heavy chain; nanoprobe system; attomoloar sensitivity
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:857
Deposited On:05 Dec 2008 15:30
Last Modified:05 Dec 2008 15:30

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