Mishra, Dinesh and Mishra, Pradyumna Kumar and Dabadghao, Sunil and Dubey, Vaibhav and Nahar, Manoj and Jain, Narendra K. (2010) Comparative evaluation of hepatitis B surface antigen-loaded elastic liposomes and ethosomes for human dendritic cell uptake and immune response. Nanomedicine: Nanotechnology, Biology and Medicine, 6 (1). 110 - 118.
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Official URL: http://www.sciencedirect.com/science/article/B7MDB...
The aim of the present study was to evaluate two vesicular carrier systems, ethosomes and elastic liposomes loaded with hepatitis B surface antigen, for in vitro qualitative and quantitative uptake by human dendritic cells (DCs) and ability to stimulate T lymphocytes. Quantitative uptake of antigen-loaded carriers was documented by flow cytometry, and internalization of the systems by the DCs was studied using spectral bioimaging. Ability of antigen-pulsed DCs to stimulate autologous peripheral blood lymphocytes and levels of TH1/TH2 cytokines were also examined using flow cytometry. Both vesicular carrier systems as antigen delivery modules and DCs as antigen-presenting cells were able to generate a protective immune response. However, ethosomes were found to have higher internalizing ability and immunogenicity in comparison with elastic liposomes. These properties of ethosomes coupled with their skin-navigating potential, make it an attractive vehicle for development of a transcutaneous vaccine against hepatitis B in preference to elastic liposomes.
|Uncontrolled Keywords:||Dendritic cells; Hepatitis B vaccine; Elastic liposomes; Ethosomes; Transcutaneous immunization|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited On:||22 Apr 2010 10:58|
|Last Modified:||22 Apr 2010 10:58|
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