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Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection

Obata, Yosuke and Ciofani, Gianni and Raffa, Vittoria and Cuschieri, Alfred and Menciassi, Arianna and Dario, Paolo and Takeoka, Shinji (2010) Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection. Nanomedicine: Nanotechnology, Biology and Medicine, 6 (1). 70 - 77.

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We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C16) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C16 liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP-positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C16 lipoplexes, the proportion of GFP-positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C16, as a model cationic amino acid–based lipid, has a high capability as a gene carrier, even for neuronal transfection.

Item Type:Article
Uncontrolled Keywords:Cationic liposomes; Amino lipids; Gene therapy; Neuronal transfection; Plasmid DNA
Subjects:Biomedical Science > Nanomedicine
ID Code:8561
Deposited By:SPI
Deposited On:22 Apr 2010 10:56
Last Modified:22 Apr 2010 10:56

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