Obata, Yosuke and Ciofani, Gianni and Raffa, Vittoria and Cuschieri, Alfred and Menciassi, Arianna and Dario, Paolo and Takeoka, Shinji (2010) Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection. Nanomedicine: Nanotechnology, Biology and Medicine, 6 (1). 70 - 77.
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Official URL: http://www.sciencedirect.com/science/article/B7MDB...
We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C16) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C16 liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP-positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C16 lipoplexes, the proportion of GFP-positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C16, as a model cationic amino acid–based lipid, has a high capability as a gene carrier, even for neuronal transfection.
|Uncontrolled Keywords:||Cationic liposomes; Amino lipids; Gene therapy; Neuronal transfection; Plasmid DNA|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited On:||22 Apr 2010 10:56|
|Last Modified:||22 Apr 2010 10:56|
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