Voisin, Muriel and Ball, Michael and O'Connell, Claire and Sherlock, Richard (2010) Osteoblasts response to microstructured and nanostructured polyimide film, processed by the use of silica bead microlenses. Nanomedicine: Nanotechnology, Biology and Medicine, 6 (1). 35 - 43.
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Official URL: http://www.sciencedirect.com/science/article/B7MDB...
The surface of polyimide ﬁlms was modiﬁed by the use of silica microspheres as microlenses to focus radiation emitted by an excimer laser. The resultant surface had both microstructures and nanostructures. Physical and chemical characterization was performed by atomic force and Fourier transform–infrared microscopy. Laser processing resulted in surfaces that had similar roughness but different component frequencies. Chemical changes were not observed with the techniques used. The response of osteoblasts to the surface was assayed by measuring their metabolic activity and the enzyme alkaline phosphatase activity, after 24 hours of growth. Cytoskeleton and expression were both investigated. Metabolic activity was similar on treated and untreated samples. Total cell number and size were increased on microstructured polymer, where speciﬁc structures were observed (protrusions). Adhesion was noted, and the actin cytoskeleton showed normal morphology. Cells on nanostructured samples had a diffuse actin network and less mature adhesions as compared with the control.
|Uncontrolled Keywords:||Microstructures; Nanostructures; Osteoblast; Biocompatibility; Adhesion|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited On:||22 Apr 2010 10:53|
|Last Modified:||22 Apr 2010 10:53|
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