Nano Archive

A reversible surface functionalized nanowire transistor to study protein-protein interactions

Lin, Shu-Ping and Pan, Chien-Yuan and Tseng, Kun-Chang and Lin, Ming-Chou and Chen, Chii-Dong and Tsai, Chia-Chang and Yu, Shu-Han and Sun, Ying-Chieh and Lin, Tsung-Wu and Chen, Yit-Tsong (2009) A reversible surface functionalized nanowire transistor to study protein-protein interactions. Nano Today, 4 (3). 235 - 243.

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Taking advantage of the reversible association–dissociation between glutathione (GSH) and glutathione S-transferase (GST), we have developed a novel method by surface-functionalizing GSH on silicon nanowire field-effect transistors (SiNW-FET) and then anchoring a particular GST-fused protein for the studies of protein–protein interactions. The reversible surface functionalization method has been proven by analytical techniques, fluorescence imaging, atomic force microscopic imaging, and electrical measurements. The reversible GSH–GST functionality on the SiNW-FET has made this sensorial device consecutively reusable and calibratable, allowing for quantitative analysis. In this work, we have demonstrated that the biologically modified SiNW-FET can be used as a screening sensor for the future studies of biomolecular associations, such as protein–protein interactions, protein–DNA interactions, protein–carbohydrate interactions, etc.

Item Type:Article
Uncontrolled Keywords:Nanowire; Field-effect transistor; Reversible surface functionalization; Protein–protein interaction
Subjects:Biomedical Science > Nanotechnology for animal health
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:8546
Deposited By:SPI
Deposited On:22 Apr 2010 10:50
Last Modified:22 Apr 2010 10:50

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