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A rapid and sensitive immunoresonance scattering spectral assay for microalbumin

Liang, Ai-Hui and Huang, Yu-Juan and Jiang, Zhi-Liang (2007) A rapid and sensitive immunoresonance scattering spectral assay for microalbumin. CLINICA CHIMICA ACTA, 383 (1-2). pp. 73-77.

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Abstract

Background: Microalbuminuria (MAU) is the earliest clinical finding for renal disease and a risk factor for hypertensive cardiovascular disease. Several methods, including enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), immunoturbidimetry (IT), immunonephelometry (IN), chemiluminescence immunoassay (CLIA), fluorescence immunoassay (FIA) and time-resolved fluorescence (TRF) have been applied for detection of MAU. However, the resonance scattering (RS) spectral assay, based on the immutioreaction and its resonance scattering effect, has not been reported. Method: In the presence of 75 mg/l polyethylene glycol (PEG), the immunoreaction of microalbumin (Malb) and its goat anti-human Malb antibody took place specifically in pH 4.4 buffer solution and aggregated to form immunocomplex particles that exhibit a strongest resonance scattering peak at 488 nm, and it was used to assay of Malb. Results: The RS intensity at 488 nm (Delta I) was proportional to the Malb concentration (C) in the range of 0.03-0.96 mg/l, the regression equation was Delta I=116.0C-2.1, the detection limit was 0.02 mg/l. Urine samples from 20 healthy subjects were assayed by this assay. The results were in agreement with those obtained with IT. Conclusion: This assay has been applied to detection of Malb in real samples, with simplicity, rapidity, high sensitivity and good selectivity. (C) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:microalbumin; immunoresonance scattering spectral assay
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:851
Deposited By:INVALID USER
Deposited On:05 Dec 2008 15:58
Last Modified:05 Dec 2008 15:58

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