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Increased mutant frequency by carbon black, but not quartz, in the lacZ and cll transgenes of Muta (TM) Mouse lung epithelial cells

Jacobsen, Nicklas Raun and Saber, Anne Thoustrup and White, Paul and Moller, Peter and Pojana, Giulio and Vogel, Ulla and Loft, Steffen and Gingerich, John and Soper, Lynda and Douglas, George R. and Wallin, Hakan (2007) Increased mutant frequency by carbon black, but not quartz, in the lacZ and cll transgenes of Muta (TM) Mouse lung epithelial cells. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 48 (6). pp. 451-461.

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Carbon black and quartz are relatively inert solid particulate materials that are carcinogenic in laboratory animals. Quartz is a human carcinogen, whereas data on carbon black are contradictory, and there are few data on mammalian mutagenesis. We determined the mutant frequency following eight repeated 72-hr incubations with 75 mu g/ml carbon black (Printex 90) or 100 mu g/ml quartz (SRM1878a) particles in the FE1 Muta (R) Mouse lung epithelial cell line. For carbon black exposed cells, the mutant frequency was 1.40-fold (95% CI: 1.22-1.58) for c// and 1.23-fold (95% CI: 1.101.37) for lacZ compared with identically passaged untreated cells. Quartz did not significantly affect the mutant frequency. Carbon black also induced DNA strand breaks (P = 0.02) and oxidized purines (P = 0.008), as measured by the Comet assay. Quartz induced marginally more oxidized purines, but no change in strand breaks. We detected five (phenanthrene, flouranthene, pyrene, benzo[a]anthracene, and chrysene) of the 16 EPA priority, polycyclic aromatic hydrocarbons (PAHs) in an extract of carbon block. The detected PAHs are only weakly mutagenic compared with benzo[a]pyrene, and were present in very low amounts. In conclusion, carbon black was weakly mutagenic in vitro at the c// and lacZ loci. It also induced DNA strand breaks and oxidized DNA bases, More studies are essential for understanding the biological significance of these findings, and clearly documenting DNA sequence changes. The results do not necessarily imply that other carbonaceous nano materials are genotoxic.

Item Type:Article
Uncontrolled Keywords:comet assay; FE1 MML; in vitro; mutants; oxidative damage
Subjects:NanoSafety > Environment, health and safety aspects of nanotechnology
ID Code:850
Deposited On:05 Dec 2008 16:08
Last Modified:05 Jan 2009 15:23

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