Usenko, Crystal Y. and Harper, Stacey L. and Tanguay, Robert L. (2008) Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish. Toxicology and applied pharmacology, 229 (1). pp. 44-55. ISSN 0041-008X
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Due to its unique physicochemical and optical properties, C60 has raised interest in commercialization for a variety of products. While several reports have determined this nanomaterial to act as a powerful antioxidant, many other studies have demonstrated a strong oxidative potential through photoactivation. To directly address the oxidative potential of C60, the effects of light and chemical supplementation and depletion of glutathione (GSH) on C60-induced toxicity were evaluated. Embryonic zebrafish were used as a model organism to examine the potential of C60 to elicit oxidative stress responses. Reduced light during C60 exposure significantly decreased mortality and the incidence of fin malformations and pericardial edema at 200 and 300 ppb C60. Embryos co-exposed to the glutathione precursor, N-acetylcysteine (NAC), also showed reduced mortality and pericardial edema; however, fin malformations were not reduced. Conversely, co-exposure to the GSH synthesis inhibitors, buthionine sulfoximine (BSO) and diethyl maleate (DEM), increased the sensitivity of zebrafish to C60 exposure. Co-exposure of C60 or its hydroxylated derivative, C60(OH)(24), with H2O2 resulted in increased mortality along the concentration gradient of H2O2 for both materials. Microarrays were used to examine the effects of C60 on the global gene expression at two time points, 36 and 48 h post fertilization (hpf). At both life stages there were alterations in the expression of several key stress response genes including glutathione-S-transferase, glutamate cysteine ligase, ferritin, alpha-tocopherol transport protein and heat shock protein 70. These results support the hypothesis that C60 induces oxidative stress in this model system.
|Additional Information:||Article Outline Introduction Methods Solution preparation General exposure protocol Dark exposure Co-incubation with GSH level-modifying chemicals Cellular death assays Global gene expression analysis Custom array chip Isolation of RNA Processing Data analysis qPCR Statistical analysis Results Light activation of C60 Chemical supplementation of GSH and ascorbic acid Chemical depletion of GSH Antioxidative potential of fullerenes Global gene expression PCR Discussion Acknowledgements References|
|Uncontrolled Keywords:||Fullerene; Photoactivation; Gene expression|
|Subjects:||Biomedical Science > Nanotechnology for animal health|
NanoSafety > Environment, health and safety aspects of nanotechnology
|Deposited By:||Lesley Tobin|
|Deposited On:||29 Oct 2008 16:47|
|Last Modified:||27 Mar 2009 17:58|
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