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Assembly of skeletal muscle cells on a Si-MEMS device and their generative force measurement

Shimizu, Kazunori and Sasaki, Hikaru and Hida, Hirotaka and Fujita, Hideaki and Obinata, Kei and Shikida, Mitsuhiro and Nagamori, Eiji (2010) Assembly of skeletal muscle cells on a Si-MEMS device and their generative force measurement. Biomedical Microdevices, 12 (2). pp. 247-252.

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Abstract

We have fabricated a simple Si-MEMS device consisting of a microcantilever and a base to measure active tension generated by skeletal muscle myotubes derived from murine myoblast cell line C2C12. We have developed a fabrication process for integration of myotubes onto the device. To position myotubes over the gap between the cantilever and the base without damage due to mechanical peeling or the use of an enzymatic reaction, we cultured myotubes on poly-N-isopropylacrylamide (PNIPAAm) as a sacrifice layer. By means of immune staining of α-actinin, it was confirmed that a myotube micropatterned onto the device bridged the gap between the cantilever and the base. After 7d differentiation, the myotube was actuated by electrical stimulation. The active tension generated by the myotube was evaluated by measuring the bending of the cantilever using image processing. On twitch stimulation, the myotube on the device contracted and generated active tension in response to the electrical signals. On tetanus tension measurement, approximately 1.0 μN per single myotube was obtained. The device developed here can be used in wide area of in vitro skeletal muscle studies, such as drug screening, physiology, regenerative medicine, etc.

Item Type:Article
ID Code:8428
Deposited By:Prof. Alexey Ivanov
Deposited On:05 Dec 2010 20:31
Last Modified:06 Dec 2010 11:26

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