Mahalanabis, Madhumita and Do, Jaephil and ALMuayad, Hussam and Zhang, Jane Y. and Klapperich, Catherine M. (2010) An integrated disposable device for DNA extraction and helicase dependent amplification. Biomedical Microdevices, 12 (2). pp. 353-359.
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Here we report the demonstration of an integrated microfluidic chip that performs helicase dependent amplification (HDA) on samples containing live bacteria. Combined chip-based sample preparation and isothermal amplification are attractive for world health applications, since the need for instrumentation to control flow rate and temperature changes are reduced or eliminated. Bacteria lysis, nucleic acid extraction, and DNA amplification with a fluorescent reporter are incorporated into a disposable polymer cartridge format. Smart passive fluidic control using a flap valve and a hydrophobic vent (with a nanoporous PTFE membrane) with a simple on-chip mixer eliminates multiple user operations. The device is able to detect as few as ten colony forming units (CFU) of E. coli in growth medium.
|Deposited By:||Prof. Alexey Ivanov|
|Deposited On:||05 Dec 2010 20:31|
|Last Modified:||06 Dec 2010 11:26|
Repository Staff Only: item control page