Nano Archive

An integrated disposable device for DNA extraction and helicase dependent amplification

Mahalanabis, Madhumita and Do, Jaephil and ALMuayad, Hussam and Zhang, Jane Y. and Klapperich, Catherine M. (2010) An integrated disposable device for DNA extraction and helicase dependent amplification. Biomedical Microdevices, 12 (2). pp. 353-359.

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Abstract

Here we report the demonstration of an integrated microfluidic chip that performs helicase dependent amplification (HDA) on samples containing live bacteria. Combined chip-based sample preparation and isothermal amplification are attractive for world health applications, since the need for instrumentation to control flow rate and temperature changes are reduced or eliminated. Bacteria lysis, nucleic acid extraction, and DNA amplification with a fluorescent reporter are incorporated into a disposable polymer cartridge format. Smart passive fluidic control using a flap valve and a hydrophobic vent (with a nanoporous PTFE membrane) with a simple on-chip mixer eliminates multiple user operations. The device is able to detect as few as ten colony forming units (CFU) of E. coli in growth medium.

Item Type:Article
ID Code:8416
Deposited By:Prof. Alexey Ivanov
Deposited On:05 Dec 2010 20:31
Last Modified:06 Dec 2010 11:26

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