Wang, Xiaoliang and Zhou, Linzhu and Ma, Yongjie and Li, Xu and Gu, Hongchen (2009) Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection. Nano Research, 2 (5). pp. 365-372. ISSN 1998-0124 (Print) 1998-0000 (Online)
Official URL: http://www.springerlink.com/content/330557u5v46594...
Using magnetic nanoparticles to enhance gene transfection, a recently developed technique termed magnetofection, has been shown to be a powerful technology in gene delivery. The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine, which is a well known nonviral transfection agent. In this article, we report methods to control the aggregate size of polyethyleneimine-coated magnetite particles. These particles were then used to enhance transfection of green fluorescent protein (GFP) into NIH 3T3 cells in vitro. We find that the aggregate size of the particles has a great effect on their performance in magnetofection, with less aggregated magnetic particles being more effective in enhancing the gene transfection.
|Subjects:||Physical Science > Nanophysics|
Physical Science > Nano objects
Material Science > Nanochemistry
Material Science > Nanostructured materials
|Divisions:||Faculty of Engineering, Science and Mathematics > School of Physics|
Faculty of Engineering, Science and Mathematics > School of Chemistry
|Deposited On:||23 Apr 2010 06:11|
|Last Modified:||23 Apr 2010 06:11|
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