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Direct Electron Transfer of Glucose Oxidase and Glucose Biosensor Based on Nano-structural Attapulgite Clay Matrix

Xu, JM and Han, WX and Yin, QF and Song, J and Zhong, H (2009) Direct Electron Transfer of Glucose Oxidase and Glucose Biosensor Based on Nano-structural Attapulgite Clay Matrix. CHINESE JOURNAL OF CHEMISTRY , 27 (11). ISSN 1001-604X

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Abstract

The direct electrochemistry of glucose oxidase (GOD) was achieved based on the immobilization of GOD on a natural nano-structural attapulgite (ATP) clay film modified glassy carbon (GC) electrode. The immobilized GOD displayed a pair of well-defined quasi-reversible redox peaks with a formal potential (E-0') of -457.5 mV (vs. SCE) in 0.1 mol.L-1 pH 7.0 phosphate buffer solution The peak Current was linearly dependent on the scan rate, indicating that the direct electrochemistry of GOD in that case was a surface-controlled process. The immobilized glucose oxidase could retain bioactivity and catalyze the oxidation of glucose in the presence of ferrocene monocarboxylic acid (FMCA) as a mediator with the apparent Michaelis-Menten constant K-m(app) of 1.16 mmol.L-1. The electroacid catalytic response showed a linear dependence on the glucose concentration ranging widely from 5.0 x 10(-6) to 60 x 10(-4) mol.L-1 (with correlation coefficient of 0.9960) This work dernonstrated that the nano-structural attapulgite clay was a good candidate material for the direct electrochemistry of the redox-active enzyme and the construction of the related enzyme biosensors The proposed biosensors were applied to determine the glucose in blood and urine samples with satisfactory results

Item Type:Article
Subjects:Material Science > Nanochemistry
ID Code:8258
Deposited By:CSMNT
Deposited On:12 Feb 2010 15:22
Last Modified:12 Feb 2010 15:22

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