Georgelin, Thomas and Maurice, Vincent and Malezieux, Bernard and Siaugue, Jean-Michel and Cabuil, Valerie (2010) Design of multifunctionalized Î³-Fe2O3@SiO2 coreâshell nanoparticles for enzymes immobilization. Journal of Nanoparticle Research, 12 (2). pp. 675-680.
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This article deals with the first covalent grafting of an enzyme on twice functionalized Î³-Fe2O3@SiO2 coreâshell magnetic nanoparticles. First, amino-PEG functionalized nanoparticles were synthesized in order to comply with non-toxic platforms that would be stable in high concentration and would exhibit chemical groups to allow further coupling with biomolecules. This approach produces a colloidal suspension of covalently grafted enzymes that remains stable for months and mimics the enzymeâsubstrate interactions in solution. Secondly, nanoparticles synthesis and enzyme coupling process were reported and the catalytic properties of bound enzymes were measured and compared with that of the free one. These new materials appear to be useful tools for enzymatic catalysis research and may be extended to other biomolecules. Furthermore, magnetic properties of these materials open the way to separation, purification, and transport under magnetic field.
|Subjects:||Physical Science > Nanophysics|
Physical Science > Nano objects
Material Science > Nanochemistry
Material Science > Nanostructured materials
|Divisions:||Faculty of Engineering, Science and Mathematics > School of Physics|
Faculty of Engineering, Science and Mathematics > School of Chemistry
|Deposited On:||10 Feb 2010 10:02|
|Last Modified:||10 Feb 2010 10:02|
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