Nano Archive

Targeted cell adhesion on selectively micropatterned polymer arrays on a poly(dimethylsiloxane) surface

Tang, Linzhi and Min, Junhong and Lee, Eun-Cheol and Kim, Jong Sung and Lee, Nae Yoon (2010) Targeted cell adhesion on selectively micropatterned polymer arrays on a poly(dimethylsiloxane) surface. BIOMEDICAL MICRODEVICES, 12 (1). pp. 13-21. ISSN 1387-2176 (Print) 1572-8781 (Online)

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Official URL: http://www.springerlink.com/content/b6g268q44560pn...

Abstract

Herein, we introduce the fabrication of polymer micropattern arrays on a chemically inert poly(dimethylsiloxane) (PDMS) surface and employ them for the selective adhesion of cells. To fabricate the micropattern arrays, a mercapto-ester—based photocurable adhesive was coated onto a mercaptosilane—coated PDMS surface and photopolymerized using a photomask to obtain patterned arrays at the microscale level. Robust polymer patterns, 380 µm in diameter, were successfully fabricated onto a PDMS surface, and cells were selectively targeted toward the patterned regions. Next, the performance of the cell adhesion was observed by anchoring cell adhesive linker, an RGD oligopeptide, on the surface of the mercapto-ester—based adhesive-cured layer. The successful anchoring of the RGD linker was confirmed through various surface characterizations such as water contact angle measurement, XPS analysis, FT-IR analysis, and AFM measurement. The micropatterning of a photocurable adhesive onto a PDMS surface can provide high structural rigidity, a highly–adhesive surface, and a physical pathway for selective cell adhesion, while the incorporated polymer micropattern arrays inside a PDMS microfluidic device can serve as a microfluidic platform for disease diagnoses and high-throughput drug screening.

Item Type:Article
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanobiotechnology
ID Code:8191
Deposited By:M T V
Deposited On:12 Feb 2010 13:58
Last Modified:12 Feb 2010 13:58

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