Nano Archive

Electroporation based on hydrodynamic focusing of microfluidics with low dc voltage

Zhu, Tao and Luo, Chunxiong and Huang, Jianyong and Xiong, Chunyang and Ouyang, Qi and Fang, Jing (2010) Electroporation based on hydrodynamic focusing of microfluidics with low dc voltage. BIOMEDICAL MICRODEVICES, 12 (1). pp. 35-40. ISSN 1387-2176 (Print) 1572-8781 (Online)

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Official URL: http://www.springerlink.com/content/ggn35mu6103824...

Abstract

Microfluidics-based cell electroporation has many advantages in delivering small molecules into cells. In this study, hydrodynamic focusing of fluids with different conductivities has been used for high through-put cell electroporation at low voltage (<3 V) of continuous direct current (dc) power. Simulation results showed that an input voltage of only 1.5 V could generate an electric field intensity of about 1.17 kV cm−1 across the cell suspension flow in the squeezed area. The electropermeation of yeast cell was observed, showing a permeabilization percentage up to 70%.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
Physical Science > Nanoelectronics
ID Code:8189
Deposited By:M T V
Deposited On:12 Feb 2010 14:03
Last Modified:12 Feb 2010 14:03

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