Nano Archive

Nanostructured conducting polymer based reagentless capacitive immunosensor

Bandodkar, Amay Jairaj and Dhand, Chetna and Arya, Sunil K. and Pandey, M. K. and Malhotra, Bansi D. (2010) Nanostructured conducting polymer based reagentless capacitive immunosensor. BIOMEDICAL MICRODEVICES, 12 (1). pp. 63-70. ISSN 1387-2176 (Print) 1572-8781 (Online)

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Nanostructured polyaniline (PANI) film electrophoretically fabricated onto indium-tin-oxide (ITO) coated glass plate has been utilized for development of an immunosensor based on capacitance change of a parallel plate capacitor (PPC) by covalently immobilizing anti-human IgG (Anti-HIgG) using N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. These fabricated PANI/ITO and Anti-HIgG/PANI/ITO plates have been characterized using scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry and Fourier transform infra-red studies. The capacitance measurements indicate that dielectric medium of this biologically modified PPC (Anti-HIgG/PANI/ITO) is sensitive to HIgG in 5 − 5 × 105 ng mL−1 range and has lower detection limit of 1.87 ng mL−1. The observed results reveal that this Anti-HIgG modified PPC can be used as a robust, easy-to-use, reagentless, sensitive and selective immunosensor for estimation of human IgG.

Item Type:Article
Subjects:Material Science > Functional and hybrid materials
Biomedical Science > Nanobiotechnology
ID Code:8185
Deposited By:M T V
Deposited On:12 Feb 2010 14:11
Last Modified:12 Feb 2010 14:11

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