Chueh, Bor-han and Zheng, Ying and Torisawa, Yu-suke and Hsiao, Amy Y. and Ge, Chunxi and Hsiong, Susan and Huebsch, Nathaniel and Franceschi, Renny and Mooney, David J. and Takayama, Shuichi (2010) Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device. BIOMEDICAL MICRODEVICES, 12 (1). pp. 145-151. ISSN 1387-2176 (Print) 1572-8781 (Online)
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This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited By:||M T V|
|Deposited On:||12 Feb 2010 14:28|
|Last Modified:||12 Feb 2010 14:28|
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