Nano Archive

Biomolecular Nano-Flow-Sensor to Measure Near-Surface Flow

Lee, Sang-Wook and Kinoshita, Haruyuki and Noji, Hiroyuki and Fujii, Teruo and Yamamoto, Takatoki (2009) Biomolecular Nano-Flow-Sensor to Measure Near-Surface Flow. Nanoscale Research Letters, 5 (2). pp. 296-301.

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We have proposed and experimentally demonstrated that the measurement of the near-surface flow at the interface between a liquid and solid using a 10 nm-sized biomolecular motor of F1-ATPase as a nano-flow-sensor. For this purpose, we developed a microfluidic test-bed chip to precisely control the liquid flow acting on the F1-ATPase. In order to visualize the rotation of F1-ATPase, several hundreds nanometer-sized particle was immobilized at the rotational axis of F1-ATPase to enhance the rotation to be detected by optical microscopy. The rotational motion of F1-ATPase, which was immobilized on an inner surface of the test-bed chip, was measured to obtain the correlation between the near-surface flow and the rotation speed of F1-ATPase. As a result, we obtained the relationship that the rotation speed of F1-ATPase was linearly decelerated with increasing flow velocity. The mechanism of the correlation between the rotation speed and the near-surface flow remains unclear, however the concept to use biomolecule as a nano-flow-sensor was proofed successfully.

Item Type:Article
Uncontrolled Keywords:Microfluidics - Near-surface - F1-ATPase - Micro-PIV - Flow-sensor
Subjects:Biomedical Science > Nanobiotechnology
ID Code:8165
Deposited By:Lesley Tobin
Deposited On:12 Feb 2010 12:27
Last Modified:12 Feb 2010 12:27

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