Lee, Sang-Wook and Kinoshita, Haruyuki and Noji, Hiroyuki and Fujii, Teruo and Yamamoto, Takatoki (2009) Biomolecular Nano-Flow-Sensor to Measure Near-Surface Flow. Nanoscale Research Letters, 5 (2). pp. 296-301.
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Official URL: http://www.springerlink.com/content/k231182v82532h...
We have proposed and experimentally demonstrated that the measurement of the near-surface flow at the interface between a liquid and solid using a 10Â nm-sized biomolecular motor of F1-ATPase as a nano-flow-sensor. For this purpose, we developed a microfluidic test-bed chip to precisely control the liquid flow acting on the F1-ATPase. In order to visualize the rotation of F1-ATPase, several hundreds nanometer-sized particle was immobilized at the rotational axis of F1-ATPase to enhance the rotation to be detected by optical microscopy. The rotational motion of F1-ATPase, which was immobilized on an inner surface of the test-bed chip, was measured to obtain the correlation between the near-surface flow and the rotation speed of F1-ATPase. As a result, we obtained the relationship that the rotation speed of F1-ATPase was linearly decelerated with increasing flow velocity. The mechanism of the correlation between the rotation speed and the near-surface flow remains unclear, however the concept to use biomolecule as a nano-flow-sensor was proofed successfully.
|Uncontrolled Keywords:||Microfluidics - Near-surface - F1-ATPase - Micro-PIV - Flow-sensor|
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited By:||Lesley Tobin|
|Deposited On:||12 Feb 2010 12:27|
|Last Modified:||12 Feb 2010 12:27|
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