Villiers, Christian L. and Freitas, Heidi and Couderc, Rachel and Villiers, Marie-Bernadette and Marche, Patrice N. (2009) Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions. Journal of Nanoparticle Research, 12 (1). pp. 55-60. ISSN 1388-0764 (Print) 1572-896X (Online)
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Official URL: http://www.springerlink.com/content/00l7112t51q244...
The effect of manufactured gold nanoparticles (NPs) on the immune system was analysed through their ability to perturb the functions of dendritic cells (DCs), a major actor of both innate and acquired immune responses. For this purpose, DCs were produced in culture from mouse bone marrow progenitors. The analysis of the viability of the cells after their incubation in the presence of gold NPs shows that these NPs are not cytotoxics even at high concentration. Furthermore, the phenotype of the DC is unchanged after the addition of NPs, indicating that there is no activation of the DC. However, the analysis of the cells at the intracellular level reveals important amounts of gold NPs amassing in endocytic compartments. Furthermore, the secretion of cytokines is significantly modified after such internalisation indicating a potential perturbation of the immune response.
|Uncontrolled Keywords:||Nanoparticles - Gold - Immune system - Dendritic cells - Cytotoxicity - Cytokine - Nanomedicine - EHS|
|Subjects:||Biomedical Science > Nanobiotechnology|
NanoSafety > Environment, health and safety aspects of nanotechnology
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
|Deposited On:||12 Mar 2010 15:55|
|Last Modified:||12 Mar 2010 15:55|
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