Maeda, Rui and Noiri, Eisei and Isobe, Hiroyuki and Homma, Tatsuya and Tanaka, Tamami and Negishi, Kousuke and Doi, Kent and Fujita, Toshiro and Nakamura, Eiichi (2008) A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertension research : official journal of the Japanese Society of Hypertension, 31 (1). pp. 141-51. ISSN 0916-9636
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Official URL: http://www.jstage.jst.go.jp/article/hypres/31/1/14...
A water-soluble fullerene vesicle based on the Buckminsterfullerene molecule (Ph(5)C(60)K, denoted as PhK) was explored to determine its effects on anti-oxidation of human umbilical endothelial cells (HUVEC) exposed to exogenous and endogenous reactive oxygen species (ROS). Hydrogen peroxide 0.05-0.25 mmol/L remarkably reduced the cellular viability of HUVEC. This reduction in viability was markedly improved when PhK 0.01-1 micromol/L was added simultaneously to the culture medium. The reduction of viability in HUVEC induced by angiotensin II (AII) 10(-9) to 10(-7) mol/L was improved by pretreatment with PhK 0.1 or 10 micromol/L 12 h before AII stimulation. The ROS indicator CM-H(2)DCFDA demonstrated the efficacy of PhK 1 or 10 micromol/L in decreasing AII-induced ROS production to the level induced by the AII receptor blocker RNH-6470 20 micromol/L. The AII-induced peroxynitrite formation, as gauged using hydroxyphenyl fluorescein as a probe, was alleviated significantly by either pretreatment with PhK 0.1 or 1 micromol/L. Electron microscopy revealed intracellular localization of PhK in HUVEC after 12 h incubation. The PhK decreased the AII-induced apoptosis and lipid peroxidation processes as revealed by hexanoyl-lysine adduct formation. These observations show that the PhK water-soluble fullerene vesicle is promising as a compound controlling not only exogenous ROS, but also endogenous AII-mediated pathophysiological conditions.
|Uncontrolled Keywords:||reactive oxygen species, endothelial cells, nano-compound, apoptosis|
|Subjects:||Biomedical Science > Nanoscale biological processes|
Biomedical Science > Nanotechnology for human health
|Deposited By:||Lesley Tobin|
|Deposited On:||08 Jan 2009 11:53|
|Last Modified:||08 Jan 2009 11:53|
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