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A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells.

Maeda, Rui and Noiri, Eisei and Isobe, Hiroyuki and Homma, Tatsuya and Tanaka, Tamami and Negishi, Kousuke and Doi, Kent and Fujita, Toshiro and Nakamura, Eiichi (2008) A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertension research : official journal of the Japanese Society of Hypertension, 31 (1). pp. 141-51. ISSN 0916-9636

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Official URL: http://www.jstage.jst.go.jp/article/hypres/31/1/14...

Abstract

A water-soluble fullerene vesicle based on the Buckminsterfullerene molecule (Ph(5)C(60)K, denoted as PhK) was explored to determine its effects on anti-oxidation of human umbilical endothelial cells (HUVEC) exposed to exogenous and endogenous reactive oxygen species (ROS). Hydrogen peroxide 0.05-0.25 mmol/L remarkably reduced the cellular viability of HUVEC. This reduction in viability was markedly improved when PhK 0.01-1 micromol/L was added simultaneously to the culture medium. The reduction of viability in HUVEC induced by angiotensin II (AII) 10(-9) to 10(-7) mol/L was improved by pretreatment with PhK 0.1 or 10 micromol/L 12 h before AII stimulation. The ROS indicator CM-H(2)DCFDA demonstrated the efficacy of PhK 1 or 10 micromol/L in decreasing AII-induced ROS production to the level induced by the AII receptor blocker RNH-6470 20 micromol/L. The AII-induced peroxynitrite formation, as gauged using hydroxyphenyl fluorescein as a probe, was alleviated significantly by either pretreatment with PhK 0.1 or 1 micromol/L. Electron microscopy revealed intracellular localization of PhK in HUVEC after 12 h incubation. The PhK decreased the AII-induced apoptosis and lipid peroxidation processes as revealed by hexanoyl-lysine adduct formation. These observations show that the PhK water-soluble fullerene vesicle is promising as a compound controlling not only exogenous ROS, but also endogenous AII-mediated pathophysiological conditions.

Item Type:Article
Uncontrolled Keywords:reactive oxygen species, endothelial cells, nano-compound, apoptosis
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanotechnology for human health
ID Code:81
Deposited By:Lesley Tobin
Deposited On:08 Jan 2009 11:53
Last Modified:08 Jan 2009 11:53

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