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Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

Gao, Fuping and Cai, Yuanyuan and Zhou, Jing and Xie, Xiaoxue and Ouyang, Weiwei and Zhang, Yuhui and Wang, Xufei and Zhang, Xiaodong and Wang, Xiaowen and Zhao, Lingyun and Tang, Jintian (2010) Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments. Nano Research, 3 (1). pp. 23-31. ISSN 1998-0124 (print version) ISSN: 1998-0000 (electronic version)

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Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe3O4 particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.

Item Type:Article
Subjects:Physical Science > Nanomagnetics
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:8099
Deposited By:M T V
Deposited On:18 Jan 2010 15:29
Last Modified:18 Jan 2010 15:29

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