Pal, Sukdeb and Kim, Min Jung and Tak, Yu Kyung and Kwon, Ho Taik and Song, Joon Myong (2009) Interdigitated microelectrode array-coupled bipolar semiconductor photodiode array (IMEA-PDA) microchip for on-chip electrochemiluminescence detection. Biomedical Microdevices, 11 (5). pp. 971-980.
This paper reports the design, fabrication and testing of a microchip wherein interdigitated microelectrode arrays (IMEA) were integrated with bipolar semiconductor photodiode array (PDA) chip to fabricate a highly compact embodiment for on-chip handling of solutions and electrochemiluminescence (ECL) detection. A 12 x 12 micro array of photodiodes, each coupled with an interdigitated microelectrode array (IMEA), an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single 2 x 2 cm(2) IC chip. Each photodiode had dimensions of 300 x 300 mu m(2) and the photodiode-to-photodiode distance was 100 mu m. The chip was successfully applied to the on-chip quantification of electro-chemiluminescing probe-labeled single stranded oligonucleotides. The minimum detectable limit at signal/noise >= 3 was found to be 5 x 10(-14) moles of oligonucleotides with a sample volume as low as 5 mu l (i.e., 10 fmole/mu l). The attractive features of the developed IMEA-PDA microchip are that a plurality of samples can be analyzed simultaneously using a chip and that for a given sample the data can be averaged from values obtained from multiple, individually addressed pixels. These in turn bring in speed and statistical confidence in analysis. The IMEA-PDA microchip system has the potential to be used as a versatile and highly compact chemical analysis tool for chemical sensing and metrology applications.
|Subjects:||Engineering > Nanotechnology applications in mechanical engineering|
|Deposited On:||25 Aug 2010 08:33|
|Last Modified:||25 Aug 2010 08:33|
Repository Staff Only: item control page